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Image Search Results
Journal: PLoS ONE
Article Title: TGF-β Blockade Reduces Mortality and Metabolic Changes in a Validated Murine Model of Pancreatic Cancer Cachexia
doi: 10.1371/journal.pone.0132786
Figure Lengend Snippet: (A) mRNA levels of Atrogin-1 and (B) MuRF1 in quadriceps muscles of mice treated with PBS or Pan02 were calculated by qPCR. (C) Similarly, levels of Atrogin-1, Myostatin, MuRF1, and β-actin in quadriceps muscle were calculated by Western blotting. (D) ZAG expression was tested in visceral adipose tissue from cohorts of mice treated with either PBS or Pan02. (E) Weight of quadriceps muscles from mouse cohorts treated with PBS or Pan02 was calculated. (F) Serum levels of MCP-1 and IL-6 were compared in PBS- and Pan02-treated mice (n = 5; *p<0.05, **p<0.01, ***p<0.001).
Article Snippet: Protein quantification was determined by the Bradford protein assay, and samples were equally loaded on 10% polyacrylamide gels (NuPage, Grand Island, NY), electrophoresed at 200V, electrotransferred to PVDF membranes, and probed with antibodies to Atrogin-1 (1:500; ECM Biosciences, Versailles, KY), Myostatin (1:200; Abcam, Cambridge, MA), MuRF1,
Techniques: Muscles, Western Blot, Expressing